Abstract
Objective To investigate the effect of radiation on the expressions of RANKL and OPG in osteoblasts in order to disclose the molecular mechanism of bone injury induced by ionizing radiation. Methods The osteoblasts were differentiated from MC3T3-E1 cells. After 2 or 4 Gy137 Cs T- irradiation, the mRNA a1nd protein expression levels of RANKL and OPG of osteoblast precursor and osteoblast were detected by real-time PCR and Western blot. Results The expressions of RANKL mRNA （t = 5.41, P 〈 0.05） and protein （t = 68.37, P 〈 0. 01 ） were up-regulated after 4 Gy irradiation, while the expressions of OPG mRNA （ t = 5.20,7.02, P 〈 0. 05 ） and protein （ t = 7.78,9.45, P 〈 0.05 ） were down-regulated after 2 and 4 Gy iITadiation. Conclllsions 2 and 4 Gy ionizing radiation alters RANKL/ RANK/OPG pathway in osteoblasts, which may promote the osteoclast differentiation and maturation and hence promote bone resorption of osteoclasts. Key words: Ionizing radiation; 0steoblasts; 0steoclasts; Receptor activator for NF-KBligand ; 0steoprotegerin
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
