Abstract
BSA chiral columns were prepared based on column modification method with carbonyl imidazole as cross-linker.Enantioseparation of tryptophan,pidotimod and 4-phenyl-1,3-oxazolidine-2-thione(L-ben) was obtained on the BSA chiral column by HPLC.The effects of pH values and ionic strength of mobile phase on enantioseparation performance were investigated.For L-tryptophan,the retention factor(k′) increased significantly as the pH value increased from 5.0 to 7.0.While for D-tryptophan k′ rarely changed and the resolution factor(Rs) increased from1.15 to 8.91.In contrast,the retention factor(k′) for RS-and SR-pidotimod decreased as the pH value increased from 5.0 to 7.0.For L-ben,k′ was only a slight increase when the pH value increased.The retention factors of three enantiomers fluctuated as ionic strength increased.However,Rs decreased when a buffer concentration increased.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
