Abstract
Objective To investigate the effect of intrathecal bupivacaine on sensor motor function and on expression of caspase-9 and caspase-8 in dorsal root ganglion in pregnant or nonpregnant rats.Methods Eighteen female SD rats weighting 200 g220 g and eighteen late pregnant rats weighting 350 g-400 g were used in this study.All the pregnant rats in which PE-10 catheter were successfully placed without complication were divided into 3 groups randomly(n=6):normal pregnant control group (group PN),2％ bupivacaine group (group P2B) and 4％ bupivacaine group (group P4B).All the nonpregnant rats in which PE-10 catheter were successfully placed without complication were divided into 3 groups randomly(n=6),normal nonpregnant-control group (group N),2％ bupivacaine group (group 2B) and 4％ bupivacaine group(group 4B).The rats of group PN and group N received normal saline 30 μl intrathecal.The rats of group P2B and group 2B received 2％ bupivacaine 30 μl intrathecal.The rats of group P4B and group 4B received 4％ bupivacaine 30 μl intrathecal Tail-flick test and motor function were performed at 10,20,30 min,1,2,4 h,1,2,3 d and 4 d after intrathecal administration.TF latencies were converted to the percent of maximal possible effect (MPE).Four days later,the animals were sacrificed and the dorsal root ganglion were removed for determination of mRNA using reverse transcriptive polymerase chain reaction (RT-PCR).Results MPE were different among the groups according timepoints after intrathecal.Among in different groups have ainteraction.MPE in group P2B and P4B were significantly higher compared with those in group 2B and 4B after intrathecal 2,4 h and 1 d.MF scores were significantly different after intrathecal 10 min-4 h in rats,group 2B,4B,P2B and P4B motor block time was longer than group N and PN.Rats in group 2B,4B,P2B and P4B showed significantly increase the expression of caspase-9 mRNA in dorsal root ganglion compared with those in group N and PN.Conclusions Intrathecal injection of the same concentration and dosage of bupivacaine,pregnant rats sensory,motor nerve block lasted significantly longer than nonpregnant rats.The result indicated that the sensitivity of bupivacaine increased in pregnancy rats.Bupivacaine showed significantly increase the expression of caspase-9 mRNA in dorsal root ganglion cells. Key words: Bupivacaine ; Pregnancy; Neurotoxicity; Cysteinyl aspartate specific protease-9 ; Apoptosis
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