Abstract
The effects of ionophores, which can carry alkali metal cations, on platelet aggregation were examined. At an alkaline extracellular pH, alkali metal cation/H + exchanger nigericin accelerated aggregation in K +-enriched medium, whereas it rather inhibited aggregation in Na +-enriched medium, even though the intracellular pH was only slightly alkaline. The inhibitory effect of Na + on platelet aggregation was more clearly shown with the alkali metal cation exchanger gramicidin D. The ionophore had no effect or a slightly accelerative effect on aggregation in K +-enriched medium, whereas it significantly inhibited aggregation induced by thrombin, ADP and platelet activating factor in Na +-enriched medium. Fluorescence studies on fura-2-labeled platelets revealed that in Na +-enriched medium gramicidin D inhibited agonist-induced Ca 2+ mobilization both in the presence and absence of extracellular Ca 2+. These results suggest that the intracellular Na + inhibits platelet aggregation by inhibiting Ca 2+ mobilization.
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