Effect of intracameral human cord blood-derived stem cells on lasered rabbit trabecular meshwork.

Abstract

This study aimed to investigate the effect of intracameral human cord blood stem cells on lasered rabbit trabecular meshwork. Immediately following diode laser application to the trabecular meshwork, human cord blood stem cells were injected intracamerally, in one eye of 12 albino rabbits. The other eye of ten rabbits was lasered controls and two eyes were normal controls. Rabbits were killed after 4, 8 and 12weeks. Lasered control rabbit eyes showed significant disruption of trabecular architecture, loss and pleomorphism of trabecular endothelial cells and progressive narrowing of trabecular spaces till 12weeks. In contrast, lasered eyes, concurrently injected with human cord blood stem cells, showed relatively preserved endothelial cellularity and structure of the trabecular meshwork, at all time points. Human CD34- and CD44-positive cells were identified in 7/8 eyes treated with stem cells, at 4 and 8weeks, and 2 of 3 at 12weeks. Many PKH26-labeled human cord blood cells were visible throughout the trabecular area at 4weeks. They gradually decreased in number by 8weeks, and at 12weeks, they appeared to be oriented along trabecular beams. There was a relative preservation of cellularity and architecture of the trabecular meshwork in eyes injected with human cord blood stem cells, as compared to lasered control eyes up to 12weeks, without significant inflammation. This suggests a probable role for such stem cells in eyes with glaucoma, having trabecular dysfunction.