Abstract
The effect of intestinal bacteria on formation of azoxymethane (AOM)-induced aberrant crypt foci (ACF) and DNA adducts in the rat colon was investigated. Male Sprague-Dawley rats were administered cultures ofLactobacillus acidophilus, Bifidobacterium adolescentis, Bacteroides fragilis, Escherichia coliandClostridium perfringensfor five weeks and given injections of AOM at 15 mg/kg body weight at the first and second weeks. The number of ACF five weeks after the start of the experiment was decreased in the rats treated with the cultures or culture supernatants ofL. acidophilusandC. perfringens.The half-life ofO6-methylguanine (O6-meG) in theL. acidophilusgroup was shorter than that in the GAM broth group. The half-life of 7-methylguanine did not differ among the groups. These results suggest that the metabolite(s) ofL. acidophilusandC. perfringensinhibit(s) the ACF formation in rats treated with AOM and that the inhibitory effect ofL. acidophilusis due to the enhanced removal ofO6-meG from the colon mucosal DNA.
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