Abstract
Background: Intermittent hepatic pedicle clamping is thought to cause less hepatic reperfusion injury compared with continuous clamping. The mechanisms underlying this difference are unknown. We examined the relationship between intermittent ischemia/reperfusion and the production of free radicals using electron spin resonance spectrometry. Methods: Alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone was administered to rats as a spin trap agent. Continuous clamping (15, 30, or 60 minutes) or intermittent clamping (four cycles of 15-minute ischemia and 5 or 15 minutes of reperfusion) of hepatic pedicle was carried out. After reperfusion, free radical production in the liver was measured by an electron spin resonance spectrometer, and the level of hepatic injury was evaluated by measuring liver enzyme. Results: Longer periods of ischemia increased free radical production after reperfusion. There was no significant increase in free radical production or liver enzymes when the duration of ischemia was 15 minutes. Free radical production and liver damage were significantly less severe in intermittent pedicle clamping than in continuous clamping for 60 minutes, especially when the duration of the reperfusion between four cycles of ischemia was 15 minutes. Conclusions: These results indicate that intermittent pedicle clamping lessens free radical production when compared with continuous clamping, although many free radicals are produced. (Surgery 1998;124:49-56.)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.