Abstract
Because of the suppression of 2′,6′-dihydroxyacetophenone(DHAP) to the combination of peptide with alkali metal ion and the advanced ability of 2,5-dihydroxybenzoic acid(DHB) to ionize peptide in MALDI-MS,it is illustrated in this work that the best MALDI-FT/ICRMS is obtained when matrix is composed of 10 μmol/L DHB and 15 μmol/L DHAP.The scanning electron microscope pictures of matrix with different compositions show that the crystal of matrix composed of 8 μmol/L DHB and 3 μmol/L DHAP is in a dense stratiform state that is quite different from crystal of matrix with other proportion of DHB and DHAP.It is also demonstrated that thin-layer method is the best peptide preparation method with matrix composed of 8 μmol/L DHB and 3 μmol/L DHAP among dry-droplet and sandwich method.
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