Abstract
Epigallocatechin gallate (EGCG) and flavonols are important phenolic compounds in green tea. These compounds are sensitive to thermal condition and their structural alteration results in making browning the green tea infusion. This study aimed to research the interaction between EGCG and flavonols during thermal infusion. EGCG and flavonols model solutions were prepared based on concentration in green tea infusion, and their colors appearance and attributes were analyzed in 10h by thermal treatment. Results showed that kaempferol, quercetin, and myricetin accelerated the oxidation of EGCG and made the model solution browning. HPLC analysis revealed there was an obvious shift of a broaden peak in the mixed model solution of EGCG and flavonols after thermal treatment. This broaden peak was further purified on HPLC and solid phase extraction methods to yield colored complexes. The complexes showed the maximal absorption around 424, 442, and 482nm. MS/MS analysis revealed that the complexes possessed of three components those were consistedof the interaction between EGCG and myricetin. These results indicated that the interaction between EGCG and flavonols might form complexes during thermal treatment, The complexes were contributed to make green tea infusion browning.
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