Abstract
Summary The objective of this study was to determine the effect of insemination volumes on embryo recovery in mares. Sixty-two light-horse mares were randomly assigned to 1 of 3 insemination treatments as they exhibited estrus. Mares were artificially inseminated with 100 × 10 6 progressively motile spermatozoa (pms) extended in 10, 100 or 200 ml of dried skim milk extender (E-Z Mixin). Embryo-recovery attempts were performed 6 or 7 days post-ovulation. 6 Due to recovery of a low number of embryos, a second experiment was conducted with the same mares using a greater number of progressively motile spermatozoa (250 × 10 6 pms) suspended in either 10 or 100 ml of E-Z Mixin extender. In Experiment 1, there was no difference (P>0.05) in numbers of embryos recovered between mares inseminated with 10 (40%) and 100 ml (10.0%) of extender, nor was there a difference (P>0.05) between mares inseminated with 100 (10.0%) and 200 ml (0.0%). However, there was a significant difference in embryos recovered between mares inseminated with 10 (40.0%) and 200 ml (0.0%) (P
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