Abstract
The present study evaluated the relative susceptibility of insecticides viz., imidacloprid, thiamethoxam, thiacloprid, flonicamid, clothianidin, diafenthiuron, spiromesifen, thiodicarb and chlorpyriphos against field collected population of Amrasca (S.) biguttula. Out of nine insecticides, maximum susceptibility was observed with thiamethoxam. The descending order of susceptibility was observed as thiamethoxam> thiacloprid> diafenthiuron> spiromesifen> imidacloprid> clothianidin> flonicamid> thiodicarb> chlorpyriphos. Based on the relative toxicity value it was observed that the insecticides such as chlorpyriphos, thiodicarb, flonicamid and clothianidin were 14.04, 12.01, 9.43 and 9.41x, respectively less toxic as compared to thiamethoxam. The detoxification enzyme assay revealed that the activity of esterase was high in thiamethoxam and thiacloprid exposed leafhopper, while cytochrome p450 activity was high in spiromesifen, thiamethoxam and thiacloprid exposed ones. Elevated level of esterase and cytochrome p450 in the insecticide exposed leafhoppers indicates the probability of insecticide resistance development.
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