Effect of Insect Exclusion and Microbial Perturbation on Piglet Mass Loss and Total Body Score

Abstract

Recent conceptual and empirical developments in decomposition research have highlighted the intricate dynamics within necrobiome communities and the roles of various decay drivers. Yet the interactions between these factors and their regulatory mechanisms remain relatively unexplored. A comprehensive understanding of this facet of decomposition science is important, given its broad applicability across ecological and forensic disciplines, and current lack of research which investigates the inter-dependencies between two critical components of the necrobiome (the microbiome and insect activity), and the consequences of this interdependency on mass loss and total body score. Here we investigated the relationships among these key aspects of the decay process. We experimentally manipulated these variables by physically excluding insects and chemically perturbing the external microbiome of piglet (Sus scrofa) carcasses and quantified the effects on mass loss and total body score, as well as insect pre-appearance interval and colonisation. We found that piglets in the insect excluded and microbially perturbed treatment groups exhibited a significant delay in reaching 50+% of mass loss compared with control piglets with insect access and intact microbiome. However, only remains with insects excluded displayed a significantly slower rate of total mass loss throughout the majority of the experiment and remained a significantly higher mass at the endpoint of 11,000 accumulative degree hours. Additionally, all insect excluded and microbially perturbed treatment groups displayed significantly lower total body scores compared to control piglets at corresponding time points. We also observed a significant delay in insect pre-appearance interval and colonisation for piglets with perturbed microbiomes compared to control piglets. Our findings demonstrate the significance of interacting components of the necrobiome, and the power of manipulative experiments in revealing causal relationships between biota and decomposition rates. These considerations are paramount for developing accurate post-mortem interval estimations and a comprehensive understanding of ecological processes during decomposition.