Effect of inorganic phosphate and Ca2+ and Mg2+ on the in vitro lysis of bovine erythrocytes

Abstract

Bovine erythrocytes became lysed in a dose-response fashion due to the presence of various concentrations of phosphates and due to the divalent cations Ca2+ and Mg2+ in a 150 mM NaCl solution. The phosphates and these dications were efficient already at the micromolar range, at 37 degrees C when the incubation period was prolonged to at least 14 hours. Autologous serum inhibited this late type of lysis when its dilution was 1:32 or lower, but increased it when it was diluted to 1:128 or higher. Haemolysate at 0.004 mM increased, ouabain was neutral, glucose at 0.1 mM and deviation from pH 7.5 delayed and EDTA at 0.1 mM albumin at 0.05 mM and iodoacetamide at 0.1 mM completely inhibited this phosphate and the Ca2+-, Mg2+-dependent lysis.