Effect of Inonotus obliquus polysaccharide on composition of the intestinal flora in mice with acute endometritis.

Binhong Hu,Wenxuan Zhang,Yichuan Ma,Rong Zhang,Yuqing Dong,Yuanyue Luo,Jingyu Pu,Xin Deng,Wenjing Zhou,Songqing Liu,Xianhua Fu,Brenda A Wilson,Luyao Li

doi:10.1371/journal.pone.0259570

Abstract

Inonotus obliquus Polysaccharide (IOP) is a large molecule extracted from Inonotus obliqus, a medicinal fungus, which has a wide range of biological activities and has been shown to be associated with inflammation. The purpose of this study is to investigate whether IOP can help to reduce acute endometritis by regulating intestinal flora. We observed pathological changes in mice with endometritis following treatment with IOP and evaluated changes in the levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α), and further studied the effects of IOP on the intestinal flora of endometritis mice using 16S rRNA high-throughput sequencing. The results showed that IOP improved the condition of uterine tissues and reduced the release of pro-inflammatory cytokines. Meanwhile, the 16S rRNA sequencing results showed that IOP could regulate the changes in intestinal microflora at the level of genera, possibly by changing the relative abundance of some genera.

Highlights

Endometritis is an infectious uterine disease that is closely related to infertility [1, 2]
Endometritis is a bacterial uterine disease that occurs in women and female animals such as cows and sows, affecting quality of life in women and modern agricultural production [2, 37]
Effect of Inonotus obliquus polysaccharide on acute endometritis swollen and eosinophilic after LPS induction which was significantly improved by Inonotus obliquus Polysaccharide (IOP) treatment

Summary

Introduction

Endometritis is an infectious uterine disease that is closely related to infertility [1, 2]. Endometritis can be divided into acute, chronic and fibrosis subtypes. The histological features of acute endometritis are congenital and intrahepatic polynuclear infiltration [1]. The cause of acute endometritis is bacterial infection [3], including Escherichia coli, Staphylococcus aureus, or Streptococcus Lipopolysacchride (LSP) in the cell wall of Gram-negative bacteria plays an important role in inflammation [4,5,6]. There are rich interactions between microorganisms and hosts [7]. Large numbers of bacteria form a microbial barrier to protect the intestinal tract in order

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