Abstract
The comparative studies on obtaining viable but nonculturable cells (VBNC) of two bacteriocin-producing strains of Lactococcus lactis ssp. lactis of different origins and activity levels under carbohydrate starvation stress have been performed. Two different types of inoculum were applied: P1, unwashed from culture liquid cells, and P2, twice washed with normal 0.9% saline cells. The P1 inoculum shifted to non-culturability faster than P2. During the whole experiment we studied changes in such strain population characteristics as total number of cells, total number of viable cells, number of colony-forming units, number of VBNC (from the nisin-producing activity), and ratio of viable to dead cells in population (using Live/Dead® (Baclight™) staining kit). After 1 year of incubation culturability decreased by 3—6 orders of magnitude in either of populations, and P1 inoculum VBNC demonstrated considerable reduction of cell size. After a year of culturing, the bacteriocin activity of P2 cells was up to 19 times higher than of P1 cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
