Abstract
Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P<0.01), but not at 0, 15, or 60 min after training, and did not affect glutamate binding in membranes obtained from the parietal cortex. These results are consistent with previous evidence for the involvement of glutamatergic synaptic modification in the hippocampus in the early steps of memory formation.
Highlights
Adult male Wistar rats (3 month old) from our own breeding colony were used
The animals were housed in plastic cages, with water and food ad libitum, under a 12h light/dark cycle at a constant temperature. They were divided into three experimental groups: group 1: the animals were left in their home cages until they were sacrificed by decapitation
The animals were withdrawn from the apparatus and placed in their home cages until they were sacrificed by decapitation 0, 5, 15 or 60 min after the training session
Summary
Adult male Wistar rats (3 month old) from our own breeding colony were used. The animals were housed in plastic cages, with water and food ad libitum, under a 12h light/dark cycle at a constant temperature. The animals were withdrawn from the apparatus and placed in their home cages until they were sacrificed by decapitation 0, 5, 15 or 60 min after the training session. The resultant pellet was washed three times in 10 mM Tris/HCl buffer, pH 7.4, and centrifuged at 27,000 g for 15 min.
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