Effect of inhibitors on active transport by turtle intestinal segments.

Abstract

1. Isolated segments of turtle (Chrysemys picta) intestine were exposed to metabolic inhibitors and the effects on active transport of D-glucose (4.5 mg./100 ml.) were studied.2. Phlorizin, iodoacetate and ouabain were potent inhibitors: 2,4-dinitrophenol, malonate, cyanide and azide were less effective.3. No significant inhibition was observed when nitrogen replaced aeration of the segments, except when segments were simultaneously treated with iodoacetate.4. The energy for the sugar transport mechanism in this animal appears to come from glycolysis.