Effect of inhibition of signal transducer and activator of transcription 3 on angiogenesis of human pancreatic cancer cells in vitro

Abstract

Objective To investigate the effect and mechanism of RNA interference ( RNAi)-mediated signal transducer and activator of transcription 3 ( STAT3 ) inhibition on human pancreatic cancer cells angiogenesis in vitro.Methods RNAi was used to inhibit STAT3 gene of SW1990 cells.Methyl thiazol tetrazolium (MTT) assay and flow cytometric analysis were performed to detect cell proliferation and cell cycle of human umbilical vein endothelial cell ( HUVEC),respectively.The migration ability of HUVECs was determined by cell migration assay.Enzyme linked immunosorbent assay (ELISA) was used to detect the protein expression of the vascular endothelial growth factor (VEGF).Results Inhibition of STAT3 with RNAi significantly inhibited the proliferation of HUVECs and decreased the migration ability of HUVECs invitro.The cell growth rate at 24,48,72 h in SW1990-RNAi group was (1.19 ±0.11)％,( 1.62 ± 0.15 ) ％,( 1.95 ± 0.18 ) ％,respectively.The percentage of HUVECs at G0/G1 phase in SW1990-RNAi group was (80.95 ± 7.49)％.Moreover,the VEGF protein expression in SW1990-RNAi cells was reduced by 60％.Conclusion Inhibition of STAT3 with RNAi can significantly inhibit the angiogenesis ability of pancreatic cancer cells through down-regulating VEGF,which may provide a novel strategy in preventing the angiogenesis of pancreatic cancer. Key words: RNA interference; Signal transducer and activator of transcription 3 ; Pancreatic carcinoma; Angiogenesis