Abstract
Prostacyclin (PGI 2) generation of cultured human vascular endothelial cells (VEC) was observed coincidentally with the increase of 45Ca net influx. Ca ionophore A23187 enhanced not only PGI 2 generation and 45Ca net influx but also 45Ca efflux. PGI 2 generation was completely abolished by the pretreatment with Ca ++ immobilizer, TMB-8. A Na + ATPase inhibitor, ouabain increased 45Ca net influx, but decreased 45Ca efflux, and enhanced PGI 2 generation. These observation indicate that PGI 2 generation of VEC may be regulated by not only Ca ++ but also Na +, and it was suggested that enhanced PGI 2 generation by ouabain might be derived from the increased cytosolic Ca ++ concentration by the decreased Ca ++ efflux, and it was considered to be originated from the suppression of Na +Ca ++ exchange systems by the increased intracellular Na + concentration via inhibition of Na +K + ATPase activity by ouabain. Enhancement of PGI 2 generation of VEC by the increased ouabain like substances (OLS) in hypertension is suspected to be beneficial on the maintenance of vascular homeostasis.
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