Effect of infection with Toxoplasma gondii in mice on the expression of Crry and CD55 In peripheral blood and spleen lymphocyte

Abstract

Objective To investigate the effect on Crry and CD55 in peripheral blood cell and spleen lymphocytes of C57BL/6 mice infected with Toxoplasma gondii (T.gondii).Methods Thirty male C57BL/6 mice were randomly divided into infected group (20mice) and control group (10mice).The mouse in infected group was intraperitoneally injected with 1.0×104 of living T.gondii RH strain tachyzoites while the mouse in the control group was injected with physiological saline.Specimens were acquired at 2d and 5d after injection.Crry and CD55 in peripheral blood cells and spleen lymphocytes were measured with flow cytometry.Results ①The positive percentages of Crry and CD55 on erythrocytes were (96.17±2.86)％,(65.23±4.99)％ in the infected group after 2d infection,and (98.24±0.83)％,(68.57±5.31)％ in the control group.There was no significant difference between two groups.After 5d,the positive percentages of Crry and CD55 were (81.77±4.51)％,(51.72±8.29)％ and (97.94±0.95)％,(70.57±6.44)％.Compared to control group,the positive percentages in the infected group were decreased significantly (t=7.786,t=4.433,P＜0.01).②The positive percentages of Crry and CD55 on leukocyte were (96.74±3.76)％,(71.80±6.74)％ in the infected group after 2d infection,and (97.25±1.16)％,(74.84±3.87)％ in the control group.There was no significant difference between two groups.After 5d,the positive percentages of Crry and CD55 were (93.464±4.38)％,(59.67±6.37)％ and (97.47±1.56)％,(75.10±4.58)％respectively in two groups.Compared to control group,the positive percentages in the infected group were decreased significantly (t=2.585,t=4.792,P＜0.05,P＜0.01).③The positive percentages of Crry and CD55 on spleen lymphocytes were (97.74±1.55)％,(66.58±2.67)％ in the infected group after 2d infection,and (98.20±1.19)％,(69.98±4.62)％ in the control group.There was no significant difference between two groups.After 5d,the positive percentages of Crry and CD55 were (94.71±3.59)％,(56.86±6.98)％ and (98.24±1.63)％,(71.18±4.09)％.Compared to control group,the positive percentages of CD55 in the infected group were decreased significantly(t=4.195,P＜0.01),but the positive percentages of Crry were not decreased notably.Conclusion The infection with T.gondii may not affect rapidly on the expression of complement regulatory proteins on peripheral blood cells and spleen lymphocytes.Along with development of infection,the positive percentages of Crry and CD55 were influenced by T.gondii RH strain at various levels. Key words: Toxoplasma gondii RH strain; Hemocyte; Spleen lymphocyte; Crry; CD55