Abstract
Within 48 hours of the institution of severe phenylhydrazineinduced anaemia in mice bearing ascites tumours or generalised leukaemia, a substantial proportion of the malignant cells disappeared respectively from the peritoneal cavity or infiltrated liver. The results of radiobiological experiments permitting determination of the proportion of viable leukaemia cells which were severely hypoxic and relatively radioresistant in the livers of leukaemic mice, showed that induction of anaemia was associated with a several hundredfold increase in the proportion of such cells. The proportion of hypoxic cells was greatly reduced when the anaemic leukaemic mice were transfused with packed erythrocytes or allowed to breathe oxygen under high pressure. Similar experi - ments with solid sarcomas indicated that a high proportion of the tumour cells were hypoxic in non-anaemic mice breathing air. The hypoxic fraction was not significantly reduced when tumour-bearing mice were made severely anaemic during growth of the tumour and were later transfused. Thus, the hypoxic cells in leukaemic livers and those in solid tumours are markedly different in their capacity for oxygenation following the induction of relative hyperoxaemia.
Highlights
SUMMARY.-Within 48 hours of the institution of severe phenylhydrazineinduced anaemia in mice bearing ascites tumours or generalised leukaemia, a substantial proportion of the malignant cells disappeared respectively from the peritoneal cavity or infiltrated liver
In interpreting the experiments described it has been assumed that phenyihydrazine hydrochloride (PH) does not directly damage proliferating cells
The rate of recovery from severe anaemia induced by the drug, as shown in Fig. 1, was similar to that from purely haemorrhagic anaemia; the reproductive integrity of malignant cells from mice which had received high doses was unimpaired; and no sign attributable to damage to the intestinal mucosa or leucocytic systems was observed
Summary
SUMMARY.-Within 48 hours of the institution of severe phenylhydrazineinduced anaemia in mice bearing ascites tumours or generalised leukaemia, a substantial proportion of the malignant cells disappeared respectively from the peritoneal cavity or infiltrated liver. In cases in which obvious factors, such as haemorrhage or bone marrow replacement, can be excluded, it is probable that continuous extravasation of blood into the tumour itself is paramount This process has been demonstrated in several transplanted animal tumours using radio-labelled erythrocytes (Greenfield, Godfrey and Price, 1958). Evans and Bergsjo, 1965) are complicated by the strong possibility that anaemic cases are more likely than non-anaemic cases to harbour malignancies which are more advanced or more aggressive. Such inadvertent selection can be avoided in experimental investigation of the effect of anaemia on response to therapy, very few such studies have been made
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