Effect of Increasing Level of Barley Distillers Dried Grains with Soluble on Ruminal Methanogenesis, Enzyme Profile and Ciliate Protozoal PopulationIn Vitro

Abstract

The study was conducted to see the effect of barley distillers dried grain with soluble (BDGS) as a feed supplement on ruminal methanogenesis, enzyme profile and ciliate protozoal population in paddy straw based diet in vitro. Five different combination of paddy straw and BDGS (viz., T1:00 mg BDGS + 200 mg of paddy straw, T2: 50 mg BDGS + 150 mg paddy straw, T3: 100 mg BDGS + 100 mg paddy straw, T4: 150 mg BDGS + 50 mg paddy straw and T5: 200 mg BDGS + 00 mg paddy straw) were used for this study. BDGS contained 95.3% OM, 19.3% CP, 73.1% NDF, 28.6% ADF and 23.1% cellulose on DM basis. The results indicated that ruminal methane production gradually decreased (P<0.05) with increasing level of BDGS in the incubating substrate. The number of total ciliate protozoa as well as holotrich and spirotrich protozoa, TVFA concentration, propionic acid and microbial biomass production increased (P<0.01) with increasing level of BDGS. The activities of polysaccharide degrading enzymes e.g., carboxy-methyl cellulase, xylanase and β-glucosidase decreased (P<0.01) with inclusion of BDGS in the incubating substrate. However, activities of amylase enzyme showed the reverse trend. In vitro true dry matter and true organic matter degradibility of paddy straw also improved (P<0.01) with supplementation of BDGS. Findings of the present study suggested that incorporation of barley distillers dried grain with soluble (BDGS) in paddy straw based diet reduced ruminal metahnogenesis and improved organic matter degradibility, TVFA and propionic acid production in vitro.