Effect of increased intracellular melanin concentration on survival of human melanoma cells exposed to different wavelengths of UV radiation

Abstract

Purpose: To investigate the effect of intracellular melanin content on cell survival of G361 human melanoma cells following exposure to UV radiation. Materials and methods: Cells were cultured in medium containing elevated concentrations of L tyrosine phosphate and L glutamine to increase their melanin content. Cell survival was assessed by colony-forming ability in treated and untreated cells following exposure to 254nm germicidal UVC radiation (0-60 J m -2) , 311nm UVB radiation (0-5 kJ m -2) , or broadband UVA radiation (Sellamed 4000, maximum output between 350 and 450nm) (0-1.2 MJ m -2) . Results: Treated cells, with a 2-fold increase in total melanin concentration and an increased ratio of eumelanin to pheomelanin, were marginally more resistant than untreated control cells to cell killing by 311nm UVB radiation, but treatment had no effect on killing by germicidal UVC or broadband UVA radiation. Conclusions: There was no evidence of any photosensitizing effect of increased melanin on human melanoma cell survival following exposure to UVC, UVB or broadband UVA radiation. The slight protective effect seen following exposure to UVB radiation may have been due to increased scavenging of reactive-oxygen species, particularly by eumelanin, at this wavelength.