Abstract
When the isolated rat heart was perfused at a range of aortic pressures, left ventricular peak systolic pressure varied from 40 to 140 mmHg. The fluorescent emission at 481 nm decreased, which was interpreted as an increased myocardial tissue NAD + NADH ratio at higher pressures. Because the perfusion conditions (fasted rats, β-hydroxybutyrate 5 m m substrate) were such as to minimize glycolytic flux and because the cytoplasmic NAD pool did not change as assessed by perfusate and tissue lactate/pyruvate ratios, it was probable that the mitochondrial NAD pool had changed towards NAD +. The effect of increased perfusion pressure on the fluorescent emission was dependent on the substrate of the heart.
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