Abstract

The effect of inbreeding on pseudo-self-compatibility (PSC) was investigated in I0, I1, and I2 clones of red clover, Trifolium pratense L., at 40°C. PSC was found to decrease with inbreeding. Significant differences were found among I0 clones, among I1 clones, and among I2 clones for PSC. Significant differences in PSC within generations were found in two of eight I1 clones and one of five I2 clones. A new method of inbred line maintenance is proposed which combines the desirable features of vegetative and seed maintenance of inbred lines. Superior I1 clones with different but homozygous S genotypes and PSC values of 8 to 10% would be vegetatively increased. Clones would be isolated under high temperature field conditions and selfed seed (PSC) would produce the I2. Selfed seed from two different I1 clonal lines would then be mixed and sown to produce single-cross seed. Singlecross seed obtained from different clonal sources would be mixed and sown for the production of double-cross hybrid red clover.

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