Abstract
The effect of lactose concentration on the activity of the immobilised enzyme β-galactosidase from Aspergillus niger has been evaluated, considering future applications for the production of galactooligosaccahrides with prebiotic potential. The following enzyme was immobilized in xanthan and chitosan polyelectrolyte multilayers (PEMs) deposited by dip coating method on polylactic acid positively corona charged pads. The pads were charged in a corona discharge system, consisting of a corona electrode (needle), a grounded plate, and a metal grid placed between them. Positive 5 kV voltage was applied to the corona electrode. 1 kV voltage of the same polarity as that of the corona electrode was applied to the grid. The chitosan layers were crosslinked with sodium tripolyphosphate (Na-TPP). The enzyme showed a temperature optimum at 50 °C and a pH optimum at 5.0. The immobilization was carried out over the different adsorption time and optimum conditions were determined. These results give insights for further optimization of transgalactosydase reactions in order to produce galactooligosaccharides with specific structure and having pronounced better prebiotic properties. For the determination of the surface morphology of the investigated samples an atomic force microscope was used and root mean square roughness was obtained.
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