Effect of IL-1β based intraperitoneal immunity levels on the prognosis of intraperitoneal micrometastasis after rectal cancer operation

Abstract

Objective To investigate the effect of L-1β-mediated intraperitoneal immune function on peritoneal micrometastasis in rectal cancer. Methods Primary human peritoneal mesothelial cells were isolated and the expressions of vimentin and VWF were detected by immunofluorescence. Human peritoneal mesothelial cells and cancer cells were stimulated with different concentrations of IL-1β and IL-1β antibody. The rate of cell adhesion was detected by flow cytometry. Results The results of immunofluorescence showed that vimentin was positive and VWF was negative. With the increase of IL-1β, cell adhesion rate were increasing. When IL-1β was added, with the increase of antibody concentration, human peritoneal interstitial endothelial cells and cancer cell adhesion rate showed declining trend. When the concentration of IL-1β was 5 ng/mL, the cell adhesion rate was the highest, which was significantly higher than that of other groups (P<0.05). The adhesion rate trend of all the groups after adding IL-1β antibody was the same as that of the other groups, but the adhesion rate of all groups was decreased. The adhesion rate of 1 ng/mL IL-1β+ 0.1 ug/mL and 5 ng/mL IL-1β group+ 0.5 ug/mL antibody group were the highest, and the lowest in the negative control group (P<0.05). Conclusion Our results show that IL-1β promotes the adhesion of rectal cancer cells to peritoneal interstitial endothelial cells in a concentration-dependent manner, indicating a favorable role of IL-1β in peritoneal carcinomatosis. Key words: Colorectal neoplasms; Immunity; Micrometastasis; IL-1β; IL-1βantibody