Abstract
The effects of ifenprodil on respiratory activity in both the presence and absence of cyanide were examined using guinea pig brain mitochondria and mouse brain cytochrome oxidase preparations. Ifenprodil stimulated respiratory activity, exhibiting maximal effect at a concentration of 0.8 m m in the presence of succinate and potassium phosphate ( P i) among the various substrates, anions, and phosphonate compounds tested. The stimulatory action of ifenprodil on respiration could not be observed with frozen and thawed brain mitochondria. The stimulatory action was not affected by oligomycin but was reduced markedly by mersalyl. Dinitrophenol and flufenamic acid did not require the presence of P i for their stimulatory action on respiration. Ifenprodil abolished completely the cyanide-induced inhibition of respiration with succinate as a substrate, but elicited no significant effects against the cyanide-induced inhibition of cytochrome oxidase activity in both in vivo and in vitro experiments. The results suggest that ifenprodil seems to act on the mitochondrial membrane by accelerating P i and succinic acid transport through the membrane as well as by stimulating succinate oxidation, and that these stimulatory actions of ifenprodil may be involved in its protective effects against cyanide toxicity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
