Effect of Hypoxic Training on Fatty Acid Oxcidation in Obese Rats

Abstract

ObjectiveTo study the effects of hypoxic training on fatty acid oxcidation of obese rats.Method130 obese rats were divided into 13 groups at random:control group, normoxic training 1,2,3,4 week group, hypoxia sedentary1,2,3,4 week group, and hypoxic training 1,2,3,4 week group. The rats in training groups ran on a treadmill for 60 min a day at 26 m/min in normoxia or 21 m/min in hypoxia(13.6%), 6 days a week for 1–4 weeks. After corresponding week, Real‐time quantitative PCR was adopted to test the expression of carnitine palmitoyl transferase (CPT‐1mRNA) in gastrocnemius and liver. Lipoprotein lipase in gastrocnemius (LPLm) and adipose (LPLa) were measured using ELISA.Results1)Hypoxic training 4 week significantly reduced CPT‐1mRNA in liver more than control, and even more than normoxic training (P<0.05) ;2) CPT‐1mRNA in gastrocnemius of obese rats was increased in 3–4 week (P>;0.05), and in 4th week, CPT‐1mRNA was significantly higher in hypoxic training group compared with normoxic training and hypoxia sedentary (P< 0.05) ;3) After 4 weeks, LPLa/ LPLm was lower in hypoxic training group than normoxic training group (P<0.05).Conclusion1)Hypoxic training increased fatty acid oxcidation of skeletal muscle more than normoxic training. 2)Hypoxic training decreased fatty acid oxcidation of liver and even more than normoxic training.(Supported by NSFC: Grant No. 30971415)