Effect of Hypoxia-Inducible Factor 1α on Early Healing in Extraction Sockets.

Hyun-Chang Lim,Je-Seon Song,Sang-Kyou Lee,Daniel S Thoma,Ui-Won Jung,Mijeong Jeon

doi:10.1155/2018/8210637

Hyun-Chang Lim, Je-Seon Song + Show 4 more

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https://doi.org/10.1155/2018/8210637

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Abstract

The aim of the present study was to investigate the effect of hypoxia-inducible factor 1α (HIF1A) on the early healing (4 weeks) of extraction sockets exhibiting partial loss of the labial bone. Two extraction sockets of the maxillary incisors from each of six dogs were assigned to two treatment modalities: deproteinized bovine bone mineral (i) with 10% collagen (DBBM-C) soaked with HIF1A and covered by a collagen membrane (CM) (HIF group) or (ii) treated with DBBM-C only and covered by a CM (control group). Microcomputed tomography revealed some degree of collapse of the labial contour. The totally augmented volume and new bone volume did not differ significantly between two groups (P > 0.05). The histological analysis revealed that the apical area of the socket was mostly filled with newly formed bone, while there was less newly formed bone in the coronal area and incomplete cortex formation. The histomorphometric analysis revealed that the area of newly formed bone was significantly larger in the HIF group than the control group (12.16 ± 3.04 versus 9.48 ± 2.01 mm2, P < 0.05), while there was no significant intergroup difference in the total augmented area. In conclusion, even though DBBM-C soaked with HIF1A enhanced histomorphometric bone formation, this intervention did not demonstrate superiority in preventing ridge shrinkage compared to DBBM-C alone. Clinical relevance of these findings should be further studied.

Highlights

The interest in counteracting ridge shrinkage has increased in recent years [1], which has led to detailed investigations of so-called alveolar ridge preservation (ARP) using a variety of protocols and biomaterials [2]
Another criticism of ARP is the possibility of further augmentation at the time of implant placement [1], which is mainly due to ARP not completely preventing ridge shrinkage
The extraction sockets were randomly assigned to the following two groups: (i) treatment with deproteinized bovine bone mineral with 10% collagen (DBBM-C; Bio-Oss5 Collagen, Geistlich Pharma, Wolhusen, Switzerland) soaked with 0.2 ml of HIF1A (4 μg of HIF1A DNA was mixed with 100 μg of Hph-1-GAL4 at room temperature for 15 min, and 0.2-ml aliquots of the solutions were used) and covered by a collagen membrane (CM; BioGide5, Geistlich Pharma) (HIF group) or (ii) treatment with DBBM-C soaked with saline and covered by a CM

Summary

Introduction

The interest in counteracting ridge shrinkage has increased in recent years [1], which has led to detailed investigations of so-called alveolar ridge preservation (ARP) using a variety of protocols and biomaterials [2]. A systematic review suggested that ARP procedures might not be able to accelerate or keep up with natural healing [3] Another criticism of ARP is the possibility of further augmentation at the time of implant placement [1], which is mainly due to ARP not completely preventing ridge shrinkage. Hypoxia-inducible factor 1α (HIF1A) is able to stimulate angiogenesis by activating genes encoding proangiogenic factors [10, 11] and enhance new bone formation and bone mineral density [12,13,14] Such angiogenic-osteogenic coupling has been tested in bone fracture, osteoporosis, and distraction osteogenesis models [12, 13, 15,16,17], suggesting that HIF1A has potential in bone tissue engineering. The aim of the present study was to investigate the effect of HIF1A on healing of sockets exhibiting partial loss of the labial bone plate at the early stage in dogs

Materials and Methods

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