Effect of Hypoxia on Prostate Cancer Cell Viability in Serum and Prostate Conditioned Media from Mixed‐Intensity Exercise Trained Rats

Abstract

BackgroundResearch suggests exercise training may alter the tumor microenvironment and facilitate conventional treatment, but the effects of exercise on prostate tumor cell growth characteristics (e.g., viability) are not well understood. In human prostate cancer cells (PC‐3), long time exposure to hypoxia decreases cellular proliferation and migration. However, whether exercise training alters tumor cell viability in hypoxia is unknown. We hypothesized that serum‐supplemented media (SSM) and prostate‐conditioned media (PCM) from mixed‐intensity exercise trained (ET) rats would decease prostate cancer cell viability versus sedentary (SED) rats in hypoxia.MethodsMale Nrf2 rats (10–12 mo old) were randomly placed into an ET (n = 10) and a SED control (n = 4) group. The ET rats ran on a motorized treadmill for 5 days/week for 10–11 weeks. Blood serum and whole prostate were collected for preparation of SSM and PCM, respectively. PC‐3 cancer cells were grown in SSM or PCM from both rat groups for 24–96 h in a 1% O2 custom hypoxia chamber placed within a standard cell incubator. Cell viability was determined using MTT cell proliferation assay.ResultsThere were no significant differences in viable cell number between SED and ET groups at 24 h (0.35 ± 0.03 vs 0.36 ± 0.02 au), 48 h (0.24 ± 0.06 vs 0.29 ± 0.04 au), and 96 h (0.47 ± 0.07 vs 0.52 ± 0.04 au) incubation in PCM. No differences were observed between the SED and ET groups at any time frame when the cells were incubated in SSM.ConclusionOur data does not support an effect of exercise training on cell viability in vitro with exposure to hypoxia. It remains to be determined if the growth supporting environment of the prostate or blood serum are altered with exercise training in vivo.Support or Funding InformationAmerican Cancer Society (RSG‐14‐150‐01‐CCE)NIH (1 R15 HL137156‐01A1)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.