Abstract
Primary cultures of hepatocytes were established from sexually mature male rainbow trout ( Oncorhyncus mykiss) and treated with the bypolipidemic drugs gemfibrozil (0.25-1.25 m M), clofibric acid (2.25-3.00 m M), or ciprofibrate (0.25-1.00 m M). Significant dose-related increases in peroxisomal fatty acyl-CoA oxidase (FACO) were seen after exposure for 48 hr to clofibric acid ( P < 0.01) and ciprofibrate ( P < 0.05) but not gemfibrozil ( P = 0.08). Strong correlation was obtained between increased acyl-CoA oxidase activity and the relative amount of peroxisomal bifunctional enzyme (PBE), further supporting evidence of a proliferative effect. These preliminary studies demonstrate that peroxisomal β-oxidation can be induced in vitro in a primary rainbow trout hepatocyte system.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
