Abstract
We studied the effect of hyperphenylalaninemia onin vitroincorporation of32P into cytoskeletal proteins from cerebral cortex of rats by injectingl-phenylalanine plus α-methylphenylalanine subcutaneously from the 6th to the 14th day postpartum. Chronic hyperphenylalaninemia induced an increasedin vitrophosphorylation of the 150-kDa neurofilament subunit and tubulins present in the cytoskeletal fraction at the end of the treatment and 3 days after treatment discontinuation. In addition, whenin vitrophosphorylation of the cytoskeletal proteins from treated animals was performed in the presence of the drugs we observed a decreasedin vitroincorporation of32P into these proteins. Thus, the effect ofl-phenylalanine plus α-methylphenylalanine on the endogenous protein kinase and phosphatase activities was examined and the results demonstrated that these drugs have an inhibitory effect on calcium/calmodulin-dependent protein kinase II and protein phosphatase type 1.
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