Effect of hypercholestermic diet on the amyloid precursor protein (APP) deposition with some behavior alterations in male rats

Abstract

Hypercholesterolemic diet (HCD) is unusually high lipid levels in blood and is strongly linked to brain damage and cerebrovascular illnesses. HCD is a chronic brain disorder characterized by cognitive impairment, inflammation, β-amyloid(Aβ) deposition. The goal of this study to investigate behavioral changes caused by the HCD in male rats. The experiment employed sixteen male rats, which was randomly split into two groups, the control group (8/ group) and the treated group (8/ group), which received a 1% cholesterol daily for 28 days. Results of behavioral test showed a significant increase (P≤0.0001) in Morris water maze test (MWM) and Elevated plus-maze test /open arm in treated group compared to control group , while a significant decrease (P≤0.0001) in Elevated plus-maze test / closed arm treated group as compared to control group. Fold change comparison between the groups expressed amyloid beta precursor protein (App) gene, this shows significant downregulation of the treatment group compared with control group. We concluded that a HCD has negative behavioral alteration that are linked to gene expression changes.