Abstract

Objective To investigate the effects of hyperbaric oxygen (HBO) on the melatonin enhancement of the differentiation of gastric cancer cells SGC7901 and also to explore the possible mechanism involved. Methods The SGC7901 cells at logarithmic differentiation stage were divided into the control group, the melatonin treatment group and the HBO coupled with melatonin group. The rate of cell differentiation was detected by flow cytemetry, and endocan expression was measured by using Western blot assay. Results SGC7901 cells treated with melatonin could enhance the rate of the cells at differential stage(the rate of the melatonin treatment group was 74.35%±0.88%, while that of the control group was 73.12%±1.01%). Melatonin treatment could also up-regulate the expression level of endocan cells (the expression level of the melatonin treatment group was 2.14±0.12, while that of the control group was 1.99±0.04). However, when treated with HBO + melatonin, both the rate of the cells at the differential stage (the rate of the cells of the HBO coupled with melatonin group was 76.86%±1.13%) and the expression of endocan cells(the rate of endocan cells of the HBO coupled with melatonin group was 2.57±0.03)were further enhanced, and statistical significance could be noted, when compared with those of the control group(P<0.05). Conclusions HBO treatment could obviously enhance the effect of melatonin on the differentiation of gastric cancer cells SGC7901, the mechanism of which might be associated with the up-regulation of endocan cells by melatonin. Key words: Hyperbaric oxygen; Gastric cancer; Cancer SGC7901; Melatonin

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