Abstract
Objective To observe effect of hyperbaric oxygen (HBO) intervention on osteoporosis in rats with experimental premature ovary failure Methods Forty female Wister rats (with an age of 6 weeks) were randomly divided into 5 groups: the sham group, the deovary group, the estrin group, the HBO group and the hyperbaric air group, each consisting of 8 animals. With the exception of the sham group, all the other groups had ovariotomy bilaterally following anesthesia. One week later, the animals in the sham group and the deovary group were left there without any treatment, while the animals in the HBO group were exposed to HBO at a pressure of 0.1 MPa, one session a day for a succession of 10 days, then had one week interval every 10 sessions, with a total exposure time of 12 weeks. The animals in the hyperbaric air group were exposed to hyperbaric air at the same pressure and with the same treatment time and conditions. The animals in the estrin group received abdominal injection of estradiol benzoate once a week, for a succession of 12 weeks. After termination of the experiment, the animals of all the groups received lumbar vertebra and femora mineral density, bone biomechanics and bone morphometry detection. Results After ovariotomy, lumbar vertebra mineral density was (0.087 8±0.007 4)g/cm2 and femora mineral density was (0.114 1±0.006 7) g/cm2. Lumbar vertebra biomechanics (216.32±45.26) N and femora biomechanics (87.50±4.01) N were significantly decreased, and statistical significance could be seen, as compared with those of the sham group [ (381.58±28.75) N and (120±16.26) N] (P 0.05), but there was obviously significant difference in them, when it was compared with that of the hyperbaric air group (P>0.05). Conclusions HBO could delay the loss of bone mineral density and the reduced property of bone biomechanics in ovarectomized rats, and it had certain protective effect on the occurrence of osteoporosis after menopause. Key words: Rat; Ovary; Hyperbaric oxygen; Bone mineral density; Bone biomechanics; Osteoporosis
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