Abstract
Hydrogen sulfide (H2S) is believed to be involved in numerous physiological and pathophysiological processes, and now it is recognized as the third endogenous signaling gasotransmitter, following nitric oxide and carbon monoxide; however, the effects of H2S on inflammatory factors in acute myocardial ischemia injury in rats have not been clarified. In the present study, sodium hydrosulfide (NaHS) was used as the H2S donor. Thirty-six male Sprague Dawley rats were randomly divided into five groups: Sham, ischemia, ischemia + low-dose (0.78 mg/kg) NaHS, ischemia + medium-dose (1.56 mg/kg) NaHS, ischemia + high-dose (3.12 mg/kg) NaHS and ischemia + propargylglycine (PPG) (30 mg/kg). The rats in each group were sacrificed 6 h after the surgery for sample collection. Compared with the ischemia group, the cardiac damage in the rats in the ischemia + NaHS groups was significantly reduced, particularly in the high-dose group; in the ischemia + PPG group, the myocardial injury was aggravated compared with that in the ischemia group. Compared with the ischemia group, the levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in the serum of rats in the ischemia + medium- and high-dose NaHS groups were significantly reduced, and the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) protein in the myocardial tissues of rats was significantly reduced. In the ischemia + PPG group, the TNF-α, IL-1β and IL-6 levels in the serum were significantly increased, the expression of ICAM-1 mRNA was increased, although without a significant difference, and the expression of NF-κB was increased. The findings of the present study provide novel evidence for the dual effects of H2S on acute myocardial ischemia injury via the modulation of inflammatory factors.
Highlights
Myocardial ischemia refers to the absolute or relative lack of coronary blood supply, or transient or chronic myocardial ischemia caused by interruption to the coronary blood supply and hypoxia
A nuclear protein/plasma protein extraction kit was purchased from Beijing Chong League International Biological Gene Technology Co., Ltd. (Beijing, China); rabbit anti-rat nuclear factor κ‐light‐chain‐enhancer of activated B cells (NF‐κB) p65 polyclonal antibody was obtained from Santa Cruz Biotechnology, Inc.; rat β‐actin polyclonal antibody was obtained from Santa Cruz Biotechnology, Inc. and rat serum tumor necrosis factor‐α (TNF‐α), IL‐1β and IL‐6 ELISA detection kits were purchased from R&D Systems, Inc. (Minneapolis, MN, USA)
Compared with the sham surgery group, the TNF‐α, IL‐1β and IL‐6 serum levels in the rats were significantly elevated in the ischemia group (P
Summary
Myocardial ischemia refers to the absolute or relative lack of coronary blood supply, or transient or chronic myocardial ischemia caused by interruption to the coronary blood supply and hypoxia. This ischemia leads to metabolic disorder of myocardial cells and the accumulation of metabolites, causing myocardial injury, or even myocardial necrosis, and thereby affecting cardiac function. It has been found that H2S exerts cardiovascular effects in the cardiovascular system, such as relaxation of vascular smooth muscle, lowering blood pressure, inhibition of vascular smooth muscle cell proliferation and regulation of cardiac contractility, and is involved in pathophysiological processes, such as hypertension, pulmonary hypertension, acute myocardial infarction and ischemia/reperfusion injury.
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