Effect of hydrogen sulfide on expressions of ectodermal dysplasia-1 and kidney injury molecule-1 of renal interstitial in rats with unilateral ureteral occlusion

Abstract

Objective To investigate the effects of hydrogen sulfide(H2S) on the tubular interstitial fibrosis and on the levels of kidney injury molecule-1(KIM-1) and ectodermal dysplasia-1(ED-1) in the process of renal interstitial fibrosis in rats with unilateral ureteral occlusion(UUO). Methods Ninety-six Sprague-Dauley(SD) male rats were divided into 4 groups randomly: sham-operated group(n=24), model group(n=24), low-dose therapy group(n=24) and high-dose therapy group(n=24). The rats in the model group and treatment groups were ligated at the left ureter and UUO was induced, meanwhile, the rats in the control group were free from the left ureter ligation. Rats received sodium hydrosulfide(NaHS) in traperitoneatly(1.4 μmol/kg, twice a day), and NaHS(7.0 μmol/kg, twice a day), respectively. Rats in sham-operated group and the model group were injected intraperitoneally with identical voluminal 9 g/L saline. Eight rats in each group were sacrificed randomly at the 7 days, 14 days and 21 days, respectively. The concentration of plasma H2S was detected. Renal tubular interstitial damage and interstitial fibrosis were evaluated with routine HE staining and Masson staining under microscope, and both of them were used to evaluate the obstruction of renal histopathological changes. The expressions of ED-1 and KIM-1 were measured with immunohistochemical staining. Results 1. The pathological findings showed that the renal tubular interstitial changes were not obvious in the sham-operated group. The tubular epithelial cells in the model group and treatment groups showed swelling and vacuoles degeneration, with renal interstitial broadening, interstitial cells and extracellular matrix increasing. The renal tubular interstitial pathological injury of model group and treatment group were more serious than those in the sham-operated group at each time point(P 0.05).2. Immunohistochemical analysis showed that expressions of ED-1 and KIM-1 in renal tubular interstitices in the model group and the treatment groups were higher than those in the sham-operated group at each time point(P 0.05).3. Plasma H2S levels in model group and treatment group were lower than those in the sham-operated group at each time point(P 0.05). Conclusions H2S implements renal protection effect partly by reducing the expression of ED-1 and KIM-1 in tubule interstitices to ease tubular interstitial fibrosis. Key words: Hydrogen sulfide; Tubular interstitial fibrosis; Uniliteral ureteral obstruction; Ectodermal dyspla-sia-1; Kidney injury molecule-1