Abstract
AbstractA series of small‐scale in vitro experiments was conducted to evaluate the effectiveness of various hydrogen peroxide (H2O2)‐dependent enzyme systems, peroxidase and glucose oxidase, in improving the nutritive value of ammonia‐treated and untreated barley straw. H2O2 and peroxidase increased (P≤0.001) digestibilities when used individually, but their combined use did not influence digestibility significantly (P>0.05). The effect of H2O2 alone was greater than that of peroxidase alone. The combination of glucose and glucose oxidase to generate H2O2 produced a small but non‐significant (P>0.05) increase in the digestibility of straw. The combination of glucose oxidase and peroxidase was ineffective in improving digestibility of straw. The digestibility values of ammonia‐treated barley straw were always greater (P≤0.001) than those of untreated straw but none of the treatments showed any interaction with ammonia treatment. It is suggested that H2O2 treatment improves the digestibility of straw.
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