Abstract
The effect of H 2O 2 and catalase on isolated rat cerebellum nitric oxide (NO) synthase activity was determined by measuring the conversion of l-[ 3H]arginine to l-[ 3H]citrulline. H 2O 2 (1–5 mM) markedly increased NO synthase activity in the presence of endogenous catalase (72 ± 4 U/mL). This effect of H 2O 2 was further increased by exogenous catalase (200 U/mL). Exogenous catalase (0.1 to 1000 U/mL) by itself had no significant effect on NO synthase activity. Nitroblue tetrazolium chloride, an electron acceptor, inhibited NO synthase activity in a concentration-dependent manner. This study suggests that H 2O 2 is not directly involved in NO synthesis and that the H 2O 2/catalase stimulation of NO synthase activity may be due to the excess oxygen produced by the H 2O 2/catalase system.
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