Abstract
Objective To evaluate the effect of hydrogen on the postoperative cognitive function in aged mice. Methods Sixty aged male Kunming mice, aged 12-14 months, weighing 35-45 g, were assigned into 3 groups(n=20 each)using a random number table: sham operation group(group Sham), partial hepatectomy group(group PH)and partial hepatectomy plus hydrogen-enriched saline group(group PH-H). On preoperative day 3, during operation and on postoperative day 3, hydrogen-enriched saline 10 ml/kg was intraperitoneally injected once a day in group PH-H, and the equal volume of normal saline was intraperitoneally injected once a day in S and PH groups. The spatial probe test was performed on postoperative day 7 to evaluate the cognitive function. Ten mice were selected on postoperative days 3 and 7(after the end of the spatial probe test), and blood samples were collected for determination of serum tumor necrosis factor-alpha(TNF-α), interleukin-1beta(IL-1β)and high-mobility group box 1 protein(HMGB1)concentrations by enzyme-linked immunosorbent assay.The animals were sacrificed after blood sampling, brains were removed and hippocampi were isolated for detection of the expression of TNF-α, IL-1β and HMGB1 by Western blot. Results Compared with group S, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly decreased, the serum concentrations of TNF-α, IL-1β and HMGB1 were increased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was up-regulated in group PH(P 0.05). Compared with group PH, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly increased, the serum concentrations of TNF-α, IL-1β and HMGB1 were decreased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was down-regulated in group PH-H(P<0.05). Conclusion Hydrogen can improve the postoperative cognitive function in aged mice. Key words: Hydrogen; Postoperative complications; Cognition disorders; Aged
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