Abstract
Objective To evaluate the effect of hydrogen on autophagy during inflammatory responses following lung injury in burned mice. Methods Ninety-six clean-grade healthy male ICR mice, aged 6 weeks, weighing 20-25 g, were divided into 4 groups (n=24 each) using a random number table: sham operation group (SH group), H2 group (H2 group), burn group (B group) and burn plus H2 group (B+ H2 group). Forty percent of the total body surface was shaved with 80 g/L sodium sulfide and then exposed to a 92 ℃ scald device for 18 s in B and B+ H2 groups.Forty percent of the total body surface was shaved with 80 g/L sodium sulfide and then exposed to a scald device of skin temperature for 18 s in SH and H2 groups.Mice inhaled 2% H2 for 1 h starting from 1 and 6 h after burn in H2 and B+ H2 groups.The animals were sacrificed at 24 h after burn and lungs were removed for determination of wet/dry weight ratio (W/D ratio), expression of autophagy-related microtubule-associated protein 1 light chain 3 (LC3) (by Western blot), activity of myeloperoxidase (MPO), and contents of interleukin-6 (IL-6) and high mobility group box 1 (HMGB1) (by enzyme-linked immunosorbent assay). The ratio of LC3-Ⅱ to LC3-Ⅰexpression (LC3-Ⅱ/LC3-Ⅰ) was calculated.The bronchoalveolar lavage fluid (BALF) was collected at 24 h after burn to detect the concentrations of IL-6 and HMGB1 and to count neutrophil. Results Compared with group SH, the W/D ratio, levels of LC3-Ⅱ/LC3-Ⅰ, MPO, IL-6 and HMGB1, concentrations of IL-6 and HMGB1 in BALF and neutrophil count were significantly increased at 24 h after scald in B and B+ H2 groups (P<0.05). Compared with group B, the W/D ratio, levels of LC3-Ⅱ/LC3-Ⅰ, MPO, IL-6 and HMGB1, concentrations of IL-6 and HMGB1 in BALF and neutrophil count were significantly decreased at 24 h after scald in group B+ H2 (P<0.05). Conclusion Hydrogen can alleviate the lung injury in burned mice, and the mechanism is related to enhancing autophagy. Key words: Hydrogen; Burn; Lung; Inflammation; Autophagy
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