Effect of hydrazine derivatives on the cholinoreactive structures of the brain

Abstract

The mercurimetric titration of homogenates of the heart, the cerebral substance of the adrenal glands, the grey matter of the brain cortex, thalamus, hypothalamus (suprahypophyseal region), the midbrain (quadrigeminal tubers) of rats carried out in the presence of acetylcholine (1·10−4) reveals an “acetylcholine wave” pointing to the presence in these tissues of cholinoreactive protein capable of interaction with acetylcholine. Hydrazine deflvatives-tubazide and iprazide (iproniazide)-preliminarily injected to rats and directly injected into the homogenate interfere with the detection of the acetylcholine wave during titration in the presence of acetylcholine of the homogenates of the heart, midbrain and thalamus, but do not disturb the interaction of acetylcholine with the recipient protein in the hornogenates of the cerebral substance of the adrenal glands, hypothalamus, and brain cortex. The capacity of hydrazides to interfere with the interaction between acetylcholine and cholinoreceptive protein is not caused by the accumulation of catecholamines or serotonin in the brain or heart tissue, because the amines them selves, when injected into the homogenate (2·10−4) do not interfere with the detection of the “acetylcholine wave”.