Abstract
A two-stage anaerobic digestion system consisting of two continuously stirred tank reactors (CSTRs) operating at mesophilic conditions (37°C) were used to investigate the effect of hydraulic retention time (HRT) on hydrogen and methane production. The acidogenic reactor was fed with a mixture consisting of olive mill wastewater, cheese whey and liquid cow manure (in a ratio 55:40:5, v/v/v) and operated at five different HRTs (5, 3, 2, 1 and 0.75d) aiming to evaluate hydrogen productivity and operational stability. The highest system efficiency was achieved at HRT 0.75d with a maximum hydrogen production rate of 1.72L/LRd and hydrogen yield of 0.54molH2/mol carbohydrates consumed. The methanogenic reactor was operated at HRTs 20 and 25d with better stability observed at HRT 25d, whereas accumulation of volatile fatty acids took place at HRT 20d. The methane production rate at the steady state of HRT 25d reached 0.33LCH4/LRd.
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