Abstract
AbstractThe bovine corneal opacity and permeability (BCOP) assay has been proposed an an in vitro method for predicting the irritancy or toxicity of chemical substances. A loss of corneal transparency, which reduces visual acuity, can be the result of an increase in corneal hydration or direct damage to corneal tissues. The BCOP assay as currently practiced does not differentiate between these mechanisms. The purpose of this study was to determine the effect of increased hydration on transparency of the bovine cornea and to measure the hydration levels of corneas treated with compounds and substances known, from previous BCOP studies, to cause corneal opacity. Corneas were treated according to the BCOP protocol and corneal opacity was determined by measurement of light absorbance at 570 nm (A570). Corneal hydration was determined by measurement of wet and dry weights. Hydration of the intact cornea was 3.86 mg H2O/mg dry weight and A570 = 0.048. After removal of the epithelium and incubation in MEM, corne...
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