Effect of hyaluranon-rich medium for embryo transfer on IVF outcome in patients with recurrent implantation failure

Abstract

Objective: The aim of the study was to evaluate efficacy of hyaluronan-rich medium on implantation and clinical pregnancy rates in vitrified-warmed blastocyst transfers in recurrent implantation failure patients. Methods: This was a randomized controlled study conducted at Akanksha IVF Centre, New Delhi. One hundred patients with history of previous two or more implantation failures, with at least one implantation failure at the present center, were included in the study. Patients were randomly divided into two groups: study group (n = 37) had embryos transferred into 20 μL of EmbryoGlue for 10 minutes prior to transfer inside uterine cavity. In the control group (n = 31), embryos were transferred to the conventional blastocyst culture medium. Statistical analysis was performed using Statistics Package for Social Sciences. Results: The patient’s mean age, causes of infertility, serum anti-Mullerian hormone level, embryo quality and the number of transferred embryos were comparable between the study and control groups. The clinical pregnancy rate in study group was higher than control group (40.5% vs 25.8%). However, the difference was not statistically significant (P = 0.58). There was no statistically significant improvement in implantation rate (44% vs 34.1%; P = 0.32), miscarriage rate (6.6% vs 12.5%; P = 0.27) and multiple pregnancy rate (13.3% vs 12.5%; P = 0.9). Conclusion: The use of EmbryoGlue in transfer medium in the blastocyst transfer in vitrified-warmed cycles does not have any significant effect on the implantation and pregnancy rates in patients with previous implantation failure.