Abstract
Sperm Vitrification is a method for cryopreservation, without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. We developed and evaluated a new method of vitrification, using a combination of 0,25 M trehalose-sucrose. This study aimed to compare the vitrification (V) with conventional freezing protocol (CF) on several sperm parameters: motility, vitality, mitocondryal activity and DNA oxidation.
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