Abstract
Exposure to purified placental ribonuclease inhibitor caused a marked reduction in ribonuclease activity associated with intact nucleoli. Addition of the placental RNase inhibitor to a nucleolar RNA synthesizing system, prior to the addition of ribonucleoside triphosphates, resulted in a significant increase in the size but did not alter the yield of the RNA products. The effect of the RNase inhibitor on the size of distribution of in, vitro transcriptional products could be reversed by the addition of N-ethylmaleimide after transcription was terminated. The properties of the ribonnclease(s) affected by the inhibitor were indicated by its resistance to N-ethylmaleimide and EDTA inhibition and by its limited endonucleolytic cleavage of the in, vitro transcript.
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