Effect of human leukocyte antigen-F associated transcript 10 on the proliferation and apoptosis of thyroid carcinoma cells

Abstract

Objective To observe the effects of human leukocyte antigen (HLA)-F associated transcript 10 (FAT10)ΔGG, a carboxyl-terminal diglycine deficient mutant, on the proliferation and apoptosis of thyroid carcinoma cell line TPC-1. Methods Specimens of cervical carcinoma in situ and normal thyroid tissue, 10 each, were collected. The expression levels of FAT10 protein in these specimens were detected by Western blotting. Site-directed mutagenesis was applied to construct the mutant pcDNA3.0-flag-FAT10ΔGG plasmid. The TPC-1 cells were then transiently transfected with wild-type FAT10, FAT10ΔGG and empty vector (used as negative control), and the wild-type TPC-1 cells served as blank control group. The transfection efficiency of FAT10 or FAT10ΔGG was detected by Western blotting, and cell proliferation was determined by cell counting kit-8 (CCK-8) assay. Cisplatin was used to induce cells after cells were transfected for 24 h, and the cell apoptotic rate was examined by flow cytometry. Results Western blotting showed a significantly increased expression of FAT10 protein in cervical carcinoma tissues as compared with that in normal cervical tissues. Over-expression of wild FAT10 in TPC-1 cells obviously promoted cell proliferation, but this promotion was significantly inhibited in cells transfected with its diglycine mutant. As compared with blank control group [(22.7±4.2)%] and negative control group [(24.1±3.8)%], the apoptotic rate was significantly reduced in wild FAT10 group [(10.9±2.0)%, P 0.05]. Conclusion FAT10 can promote cell proliferation and inhibit cell apoptosis through its carboxyl-terminal diglycine motif, and it may play an essential role in carcinogenesis and development of cancer. Key words: Thyroid carcinoma; Human leukocyte antigen-F associated transcript 10; Apoptosis