Effect of hUHRF1 silencing on proliferation and apoptosis of human cancer cell line SKOV-3

Abstract

Objective To discuss the effects of hUHRF1 gene on proliferation and apoptosis effects of human ovarian cancer cell line SKOV-3.Methods Small interfering RNA (siRNA) for hUHRF1 gene sequence was transfected into SKOV-3 cells,Real-time quantitative polymerase chain reaction ( RTqPCR) and Western blotting assays were used to detect the mRNA and protein expression levels of hUHRF1 respectively before and after siRNA transfection.Cell counting Kit-8 (CCK-8) assay was used to investigate proliferation.Flow cytometry was used to measure apoptosis.Results mRNA expression of hUHRF1 was significantly decreased in SKOV-3 cells after the transfection of siRNA ( P ＜ 0.01 ).Relative expression of hUHRF1 protein in transfection group,negative control group and blank group was 0.72 ± 0.42,1.66 ± 0.27 and 1.62 ± 0.29 respectively.The proliferation of SKOV-3 cells was inhibited after transfection.Apoptosis rate of SKOV-3 cells in transfectiou group,negative control group and blank group was (42.320 ±4.174)％,( 17.740 ± 1.786)％ and ( 15.440 ± 1.233)％,respectively.Conclusion Using the siRNA gene silencing techniques,the expression of hUHRF1 is significantly inhibited after gene silencing in SKOV-3 cells, which can effectively inhibited SKOV-3 cell proliferation and iuduced apoptosis. Key words: Ovarion cancer; RNA interference; hUHRF1